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Cell line: | JURKAT |
DSMZ no.: | ACC 282 |
Species: | human (Homo sapiens) |
Cell type: | T cell leukemia |
Origin: | established from the peripheral blood of a 14-year-old boy with acute lymphoblastic leukemia (ALL) at first relapse in 1976 (in the original paper by Schneider et al. the cell line was labelled as JM); occasionally certain subclones (like E6-1) with somewhat divergent features may exist from various sources other than DSMZ. Exome and RNA sequence data of our JURKAT are available (see Ref 18187 and Exome sequence and RNA-Seq) |
Reference(s): | 14944, 18187 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing singly or in clumps in suspension; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine |
Subculture: | seed out at about 0.2-0.5 x 106 cells/ml; optimal split ratio of about 1:2 to 1:3 every 2-3 days; maintain at 0.5-1.5 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 25-35 hours |
Harvest: | maximal density at about 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
Immunology: | CD2+, CD3+, CD4-, CD5+, CD6+, CD7+, CD8-, CD13-, CD19-, CD34(+), cyCD3+, TCRalpha/beta+, TCRgamma/delta- ; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypotetraploid karyotype with 7.8% polyploidy - 87(78-91)<4n>XX, -Y, -Y, -5, -16, -17, -22, add(2)(p21)/del(2)(p23)x2 - sideline with additional der(5)t(5;10)(q11;p15), del(9)(p11) |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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