| | |
|---|
| Cell line: | DV-90 |
| DSMZ no.: | ACC 307 |
| Species: | human (Homo sapiens) |
| Cell type: | lung adenocarcinoma |
| Origin: | established from the pleural effusion of a 50-year-old man with lung adenocarcinoma (stage IV) in 1990; cells were described in the literature to be tumorigenic in nude mice; listed in the CCLE (ref 18232) |
| Reference(s): | 14779, 40405, 40412, 18232 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent cells growing in monolayers; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine + 1x non-essential amino acids |
| Subculture: | seed out ca. 2 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA; after seeding, cells may need 2-3 days to adhere |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 95 hours |
| Harvest: | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-diploid karyotype with 17% polyploidy - 46(41-47)<2n>X, -Y, +8, add(6)(q27) - matches published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
