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Cell line: | EFM-192B |
DSMZ no.: | ACC 308 |
Species: | human (Homo sapiens) |
Cell type: | breast carcinoma |
Origin: | established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old woman with breast adenocarcinoma; cells were described in the literature to overexpress the erB-2 gene; sister cell lines EFM-192A (DSMZ ACC 258) and EFM-192C (DSMZ ACC 311) show differences in some molecular aspects;
RNA sequence data are available (see Ref 40906 and RNA-Seq) |
Reference(s): | 15156, 40906 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epitheloid adherent cells growing as monolayers; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | subcultivation before reaching confluence; split 1:2 to 1:3 once a week using trypsin/EDTA; seed out at ca. 0.5-1.0 x 105 cells/cm2 |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 4-5 days |
Harvest: | cell harvest of ca. 2-4 x 105 cells/cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human heteroploid hypotriploid karyotype with 13% polyploidy - 62(58-62)<3n>XXX/XX, -X, +2, +4, +6, -7, -8, -8, -10, -11, -12, +14, +16, -17, -17, -17, -18, -18, -19, -20, -21, -22, +8mar, add(1)(p22-31), add(2)(q?35), del(3)(p22), add(5)(p15), del(6)(q25), add(10)(p11), add(13)(q32), add(14)(p12), add(15)(p12), der(15)t(10;15)(q11;p12), der(20)t(1;20)(q24;q12/13), der(21)t(7;21)(q11;p12)x2 - most cells carry a small ring and 1-2 large submetacentric 15p+ markers - closely related to EFM-192A - apparent ch 17 nullisomy |
Molec. Genetics: | no or weak expression of ER, PR, AR but strong expression of HER2 as determined by Western blot |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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