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| Cell line: | HPD-1NR |
| DSMZ no.: | ACC 314 |
| Species: | Syrian hamster (golden hamster) (Mesocricetus auratus) |
| Cell type: | pancreas ductal adenocarcinoma |
| Origin: | established from the pancreatic ductal adenocarcinoma of a female Syrian (Golden) hamster (Mesocricetus auratus) induced by treatment with BHP (chemical carcinogen); sister cell line of HPD-2NR (DSM ACC 293); described in the literature to be homotransplantable (i.e. in other syngeneic hamsters) and to carry a mutation in exon 1 of the c-Ki-ras oncogene |
| Reference(s): | 14784 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epithelial cells growing mostly in monolayers (about 2-5% very large cells), but also with formation of a three-dimensional grid-like structure with some round, only loosly bound cells; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | split confluent culture before reaching confluence 1:2 to 1:4 every 3-6 days using trypsin/EDTA; seed out at about 2 x 106 cells/80 cm2; cells have a high metabolic activity acidifying the culture medium; exchange spent medium every 2 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-70 hours |
| Harvest: | cell harvest of about 6-7 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mesocricetus auratus species. |
| Cytogenetics: | near triploid highly rearranged karyotype with 36% polyploidy - 60-66<3n>XX, -X - hamster markers present - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
