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Cell line: | KYSE-140 |
DSMZ no.: | ACC 348 |
Species: | human (Homo sapiens) |
Cell type: | esophageal squamous cell carcinoma |
Origin: | derived from the moderately differentiated invasive squamous cell carcinoma resected from middle intra-thoracic esophagus of a 54-year-old man prior to treatment (depth of invasion was a1); described in the literature to carry p53 mutation |
Reference(s): | 15006, 14955, 14646, 14954, 18232 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent cells growing as monolayer with the tendency to pile up; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 2-4 days using trypsin/EDTA (cells may need five or more minutes to detach from the flask; prior to using trypsin, cells should be washed twice with PBS) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 24 hours |
Harvest: | cell harvest of ca. 20-30 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid/hypotriploid karyotype with 19% polyploidy - 59(56-64)<2n>Xder(X), -Y, add(X)(p1?), del(1)(q32), add(1)(q43), i(3q), add(2)(p2?), del(3)(q22), add(4)(p11), del(4)(q11), add(5)(q1?)x1-2, ins(6;?)(q1?), add(7)(q35)x2, add(8)(q24), del(9)(p11), add(9)(q34), add(11)(p15), add(12)(q24), add(13)(p11), i(13q), add(18)(p12), add(19)(p13), inc. - many additional structural and numerical rearrangements, including multiple insertions, producing an overwhelming preponderance of marker chromosomes |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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