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| Cell line: | BFTC-905 |
| DSMZ no.: | ACC 361 |
| Species: | human (Homo sapiens) |
| Cell type: | urinary bladder transitional cell carcinoma |
| Origin: | established from a 51-year-old woman with a grade III papillary transitional cell carinoma of the urinary bladder in 1990; patient lived in a blackfoot disease endemic area in Taiwan |
| Reference(s): | 14430, 15028, 18168 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing adherently in monolayers, forming large islets; image; image; image; image |
| Medium: | 80-90% DMEM + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 2 x 106 cells/80 cm2; split culture before reaching confluency 1:3 to 1:5 once or twice a week using trypsin/EDTA (for 5-10 min) |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 60-70 hours |
| Harvest: | cell harvest of ca. 10-15 x 10 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS and 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays; bacterial contamination was eliminated with Baytril (enrofloxacin) and BM-Cyclin (tiamulin & minocycline) |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hyperdiploid/hypotriploid karyotype - 58(52-61)<3n>X/XX-X, -4, +5, -9, -13, -15, -16, -18, -21, -22, -22, +mar, add(1)(p36), i(5p), add(5)(q35), del(9)(p22), del(11)(p11), add(11)(q13), add(13)(p12), add(13)(p12), add(15)(p12), add(19)(p13) - i(5p) and loss of 9p associated with transitional cell carcinoma - matches published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
