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| Cell line: | D3 |
| DSMZ no.: | ACC 428 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | hybridoma (anti-chicken N-CAM) |
| Origin: | cell line was established by fusion of mouse NS-1 cells with spleen cells from a BALB/c mouse immunized with wheat germ agglutinin affinity purified chicken brain proteins immobilized on lectin beads; described as secreting a mouse monoclonal antibody (IgG) reacting with the cytoplasmic domain of the 140, 180 and 250 kDa glycosylated neuronal cell surface protein N-CAM, preferentially in neurons of the chicken visual system like retina, tectum and other CNS regions and PNS; also reacting with mouse, goldfish and human N-CAM in paraformaldehyde-fixed cryosections and Western blots |
| Reference(s): | 14937 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round, single cells growing in suspension and some slightly adherent; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement") |
| Subculture: | maintain at ca. 0.5 x 106 cells/ml; split saturated culture 1:5 to 1:10 every 2-3 days (some cells attach to the surface of the culture flask, but can be detached by tapping the flask); seed out at ca. 0.5 x 106 cells/ml; maximum density of ca. 1-2 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 22 hours |
| Harvest: | cell harvest of ca. 0.5-1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | secretion of a IgG2A monoclonal antibody |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine flat-moded hypertetraploid karyotype - 98-104<4n> - two centric fusion markers present |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
