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| Cell line: | MOLT-4 |
| DSMZ no.: | ACC 362 |
| Species: | human (Homo sapiens) |
| Cell type: | T cell leukemia |
| Origin: | established from the peripheral blood of a 19-year-old man with acute lymphoblastic leukemia (ALL) in relapse in 1971; sister cell line of MOLT-3 (DSMZ ACC 84). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 14767, 15217, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing in suspension, singly or in clusters; image; image; image; image |
| Medium: | 80% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-1.3 x 106 cells/ml; split ratio of ca. 1:2 to 1:3 every 2-3 days; viability may transiently decrease after thawing |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40 hours |
| Harvest: | maximal density at ca. 1.3 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD2(+), CD3(+), CD4(+), CD5+, CD6+, CD7+, CD8(+), CD13-, CD19-, CD34(+), TCRalpha/beta-,
TCRgamma/delta-, cyCD3+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypertetraploid karyotype - 89-99<4n>XXYY, +4, +7, +8, +20, +20, del(6)(q16)x2, der(7)t(7;7)(p15;q11)x2 - closely related to MOLT-3 |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
