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Cell line: | LOU-NH91 |
DSMZ no.: | ACC 393 |
Species: | human (Homo sapiens) |
Cell type: | lung squamous cell carcinoma |
Origin: | established from the lower right lung lobe removed two months after diagnosis of highly differentiated squamous cell lung carcinoma with metastasis to one lymph node (no visceral involvement) in a 64-year-old woman (with any prior therapy); listed in the CCLE (ref 18232) |
Reference(s): | 14494, 18232 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent ovoid-to-epitheloid cells growing in monolayers; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine |
Subculture: | seed out at ca. 0.5-1.0 x 106 cells/80 cm2; initially, growing may be slow; later, split confluent culture 1:2 to 1:4 every 5-10 days using trypsin/EDTA (incubation time may be 5 min or longer) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 80 hours |
Harvest: | cell harvest of ca. 1.0-1.5 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with declining hyperhaploid sideline (2%) and 9% polyploidy - 50-53<2n>XX/XXXX, +5, +5, +7, +7, -13, +17, -18, +22, +22, +1-2mar, del(4)(q27), ?add(22)(q13)x2 (major clone) - 54<2n>XXXX, +5, +5, +7, +7, +22, +22 (minor clone) - 27XX, +5, +7, +22 - matches published karyotype - unstable karyotype with recent gain of structural rearrangements |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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