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| Cell line: | LOUCY |
| DSMZ no.: | ACC 394 |
| Species: | human (Homo sapiens) |
| Cell type: | T cell leukemia |
| Origin: | established from the peripheral blood of a 38-year-old woman with T cell acute lymphoblastic leukemia, T-ALL (FAB L2) (chemotherapy-resistant, remission was never achieved) |
| Reference(s): | 14379, 15868, 16550, 16557 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round, rather small cells growing singly in suspension; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-60 hours |
| Harvest: | maximal density at ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD2 -, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 16% polyploidy - 45<2n>X, -X, del(1)(p3?2p3?4), del(5)(q14-15q34-35), t(16;20)(p1?1;q1?3) - sideline with del(6)(q23) - 1p32, 1p34 and 6q- recurrent in T-ALL - resembles published karyotype |
| Molec. Genetics: | expression of MEF2C and of a SET-NUP214 fusion gene was confirmed by RT-PCR and Western blot |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
