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Cell line: | RPMI-8226 |
DSMZ no.: | ACC 402 |
Species: | human (Homo sapiens) |
Cell type: | multiple myeloma |
Origin: | established from the peripheral blood of a 61-year-old man with multiple myeloma (IgG lambda-type) at diagnosis in 1966; described in the literature to produce and secrete only lambda light chains (but not heavy chains). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
Reference(s): | 14486, 14749, 18187 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | cells grow mostly as single cells in suspension, some cells may grow adherent (occasionally up to 50%); image; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.4-0.5 x 106 cells/ml; split saturated culture 1:2 every 3 days; cells may grow slightly adherent; cells may be detached by tapping the flask or with a rubber policeman or with TNE (40 mM TrisCl pH 7.5, 1 mM EDTA pH 7.5, 150 mM NaCl); trypsin/EDTA usually not necessary |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 60-70 hours |
Harvest: | cell harvest of ca. 1 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD3 -, CD5 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, CD80 -, CD138 +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypotriploid karyotype with 7.5% polyploidy - 62-67<3n>XXY, -1, +5, +6, +7, -8, -9, -10, +11, -12, -13, -14, +16, +18, -19, +21, -22, der(1)t(1;1)(p36.3;q11-12), add(3)(q27-29)x2, del(4)(p14p15.4), der(5;6)(q10;p10)x1-2, del(5)(q32), del(6)(q15), add(9)(p24), add(11)(p15), del(11)(q25), der(14)t(1;14)(q11-12;q32)x2, del(15)(q11.2q14)x2, add(16)(q23-24), der(17)t(?8;17)(q21.2;q25), del(22)(q13.2) - der(1), 6q-, 14q+ and 22q- associated with multiple myeloma - identified by disomic 14q+ |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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