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Cell line: | GMS-10 |
DSMZ no.: | ACC 405 |
Species: | human (Homo sapiens) |
Cell type: | glioblastoma |
Origin: | established from the malignant brain tumor of a 49-year-old man with glioblastoma in 1995 |
Reference(s): | not published |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | fibroblast-like cells growing as monolayers |
Medium: | 85-90% DMEM + 10-15% h.i. FBS + 2 mM L-glutamine |
Subculture: | seed out at ca. 0.5-1.0 x 106 cells/80 cm2 ; split confluent culture 1:3 to 1:5 every 3-6 days using trypsin/EDTA |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 50-70 hours |
Harvest: | cell harvest of ca. 5 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypertriploid karyotype with 11% polyploidy - 69-76<3n>XXYY, +Y, +2, +3, +9, -10, -12, +15, +15, -18, +20, +20, -22, +2-4mar, +1-4dmin, del(2)(p11), add(2)(q31;hsr), der(4)t(3;4)(p21;p15.3), add(4)(p1?), del(6)(q23), add(7)(q11), add(9)(p23), add(13)(p11), add(15)(p11)x3, add(19)(q13), add(21)(p11)x3 - same hsr present in mar and add(2) |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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