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| Cell line: | 697 |
| DSMZ no.: | ACC 42 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell precursor leukemia |
| Origin: | established from the bone marrow of a 12-year-old boy with acute lymphoblastic leukemia (cALL) at relapse in 1979; cells express BCL2, BCL3 and MYC mRNA; recently termed "EU-3" in the literature. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s): | 14533, 40437, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round, single cells and clusters of cells in suspension; image; image; image; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0-5-1.0 x 106 cells/ml;
maintain at 0.5-1.5 x 106 cells/ml; split saturated culture at 1:3 every 2-3 days; it may be of advantage to start in 24-well-plates |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 20-40 hours |
| Harvest: | maximal density at about 2-3 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 -, CD38 +, CD80 -, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near diploid karyotype - 46(45-48)<2n>XY, t(1;19)(q23;p13), del(6)(q21) |
| Molec. Genetics: | expression of fusion gene TCF3-PBX (E2A-PBX) confirmed by RT-PCR |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
