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| Cell line: | CL-11 |
| DSMZ no.: | ACC 467 |
| Species: | human (Homo sapiens) |
| Cell type: | colon carcinoma |
| Origin: | established from the primary tumor at the left colon (a moderately differentiated colorectal carcinoma of TNM stage 3); sister cell line of mucoid growing cell line CL-11M; cells were described as expressing tumor-associated carcino-embryonic antigen (CEA) |
| Reference(s): | 14528 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epitheloid cells growing in monolayers and forming islets; image; image; image |
| Medium: | 80% mixture of Ham's F12 + DMEM (at 1:1) + 20% h.i. FBS |
| Subculture: | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:5 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 3-4 days |
| Harvest: | cell harvest of ca. 12 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-diploid karyotype with 12% polyploidy - 46(45-47)<2n>XY, -6, +7, -17, -18, +2-3 mar, +1-4 supernumerary r/dm, add(8)(q24) - sideline with idem, +7 |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
