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Cell line: | A-10 |
DSMZ no.: | ACC 132 |
Species: | rat (Rattus norvegicus) |
Cell type: | smooth muscle |
Origin: | established from the thoracic aorta of a DB1X embryonic rat in 1976; cells were described in the literature to display properties characteristic of smooth muscle cells |
Reference(s): | 14662 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | large, epithelial-like, some fibroblast-like, adherent cells growing in monolayers; image; image; image; image; image |
Medium: | 80% DMEM + 20% h.i. FBS |
Subculture: | seed out at 1 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 once or twice a week using trypsin/EDTA; after thawing, cells may contain a significant amount of granulae; confluent cultures look more transparent |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 70-100 hours |
Harvest: | about 1-2 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
Cytogenetics: | rat karyotype |
Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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