| |
---|
Cell line: | CAT-13.9C1 |
DSMZ no.: | ACC 475 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | hybridoma (CD23) |
Origin: | described as secreting a mouse monoclonal antibody (IgG2akappa) CD23; the cell line was established in 1988 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with an EBV-transformed human B cell line from a patient with MHC class II deficiency re-expressing class II antigens |
Reference(s): | not published |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round cells, growing singly or in small clumps |
Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing) |
Subculture: | split saturated culture 1:2 every 2 days; seed out at about 0.2 x 106 cells/ml; maintain between 0.2-0.8 x 106 cells/ml |
Incubation: | at 37°C with 10% CO2 |
Doubling time: | about 48 hours |
Harvest: | maximal density of about 1.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | secretion of a IgG2a monoclonal antibody |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |