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| Cell line: | JMSU-1 |
| DSMZ no.: | ACC 505 |
| Species: | human (Homo sapiens) |
| Cell type: | urinary bladder carcinoma |
| Origin: | derived from malignant ascitic fluid of a 75-year-old man with urinary bladder carcinoma in 1988; described as being of epithelial origin with morphologically distinct cells (polygonal to spindle-shaped) and as expressing cytokeratin-19 fragment |
| Reference(s): | 14786, 14787 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | longly cells, some forming fiber-like contacts, growing adherently in monolayers and small clusters; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 2-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypertriploid karyotype with 12% polyploidy - 69-74<3n>X/XX, -Y, -Y, +1, +1, +2, -5, +9, -12, +13, -15, -15, -16, -17, +19, -22, +9mar, add(1)(q32), add(2)(q3?), del(4)(q31q33), del(6)(q25), add(7)(p11), del(8)(p22), del(9)(p22), del(10)(p12), del(10)(q22), add(11)(p12), add(13)(p11), add(16)(p13), i(17q), add(19)(p13) - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
