| |
---|
Cell line: | ALL-SIL |
DSMZ no.: | ACC 511 |
Species: | human (Homo sapiens) |
Cell type: | T cell leukemia |
Origin: | established from the peripheral blood of a 17-year-old man with T-ALL (T cell acute lymphoblastic leukemia) at relapse; also found in the literature as SIL-ALL; carries NUP214-ABL1 fusion gene |
Reference(s): | 15217, 14574 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | mostly round cells growing singly or in small clusters in suspension; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml; maximal density of ca. 1.5 x 106 cells/ml; it may be of advantange to first culture the cells after thawing in a 24-well-plate with 20% FBS |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 48 hours |
Harvest: | cell harvest of about 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, RNA hybridization, PCR assays |
Immunology: | cyCD3 +, CD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypertetraploid karyotype - 90-95<4n>XX/XXYY, +6, +8, +8, t(1;13)(p32;q32)x2, del(6)(q25)x2, del(9)(?p23p24)x2, t(10;14)(q24;q11.2)x2, add(17)(p11)x2 - sideline with idem, -20, -20 - carries t(10;14) effecting TLX1-TRD (HOX11-TCRD) juxtaposition - matches published karyotype |
Molec. Genetics: | expression of TLX1 confirmed by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |