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| Cell line: | CAL-29 |
| DSMZ no.: | ACC 515 |
| Species: | human (Homo sapiens) |
| Cell type: | urinary bladder transitional cell carcinoma |
| Origin: | established from the primary lesion of a fatally invasive, metastatic transitional cell carcinoma of the bladder (grade IV, stage T2) from an 80-year-old woman before treatment |
| Reference(s): | 14423 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells, some with spindle-like extensions growing adherently in monolayers; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-60 hours |
| Harvest: | maximal density of ca. 6.0 x 106 cells/80 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypertriploid karyotype with 8% polyploidy - 71(63-74)<3n>XX/XXX, -4, +9, -11, -13, -17, +20, +3mar, +1-6 dmin, i(3q), add(7)(q22)x1-2, del(7)(q32)x2, del(9)(p21), del(10)(p13), add(12)(p1?3), add(19)(p13) |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
