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Cell line: | COS-7 |
DSMZ no.: | ACC 60 |
Species: | monkey - African green monkey (Chlorocebus aethiops (Cercopithecus aethiops)) |
Cell type: | kidney |
Origin: | derived from CV-1, a simian cell line (cercopithecus aethiops), by transformation with an origin-defective mutant of SV-40; cells were described in the literature to support the growth of SV-40 viruses; classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS) |
Reference(s): | 14568, 18224 |
Biosafety level: | 1, GMO-S1 |
Risk assessment: | The cell line was established by transfection with a mutated simian virus 40 (SV40) exhibiting a 6 bp deletion in the replication origin. This renders the virus incompetent for replication and the cell line can thus be handled under biosafety level 1. |
Permissions and restrictions: | A, D, E |
| DSMZ Cell Culture Data: |
Morphology: | fibroblast-like cells growing as monolayers
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Medium: | 90% DMEM + 10% h.i. FBS |
Subculture: | split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2 |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 35-48 hours |
Harvest: | about 2-3 x 106 cells/80 cm2; saturation density at about 5 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Chlorocebus aethiops |
Cytogenetics: | simian hypodiploid karyotype with 25% polyploidy - 57(53-58)<2n> |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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