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Cell line: | KYO-1 |
DSMZ no.: | ACC 601 |
Species: | human (Homo sapiens) |
Cell type: | chronic myeloid leukemia in blast crisis |
Origin: | established from the peripheral blood of a 22-year-old man with chronic myeloid leukemia (CML) in myeloid blast crisis in 1981; cells carry Philadelphia-chromosome translocation |
Reference(s): | 14814 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing mostly singly or occasionally in clumps in suspension; a few cells are double-sized; image; image; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; split culture 1:2 to 1:4 every 2-3 days; maintain at ca. 0.2-0.8 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 40-50 hours |
Harvest: | cell harvest of ca. 1 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3-, CD11b-, CD13-, CD14-, CD15-, CD19-, CD33+, CD34-, CD41-, CD42-, CD71+; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypotetraploid karyotype; 83-90<4n>XX, -Y, -Y, +5, +8, -9, -9, +11, +11, +15, -16, -17, -17, -18, -18, +22, +22, +2mar; t(3;12)(p12.2;p11-12) x2, der(5)t(5;6)(p15;?q22)t(6;12)(q2?;?)ins(6;12)(q2?;?), del(6)(q22) x2, der(8)t(8;9)(q25;p11-12) x2, der(9)add(9)(p11-12)t(9;22)(q34;q11) x2,
add(11)(q11) x2, der(12)t(6;12)(?;?) x2, der(15)t(15;22)(p11;q11) x2, der(17)t(17;17)(p12-13;q23) x2, der(22)t(9;22)(q34;q11) x4;
carries t(9;22) effecting BCR-ABL1 rearrangement;
resembles published karyotype |
Molec. Genetics: | expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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