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Cell line: | RAMOS |
DSMZ no.: | ACC 603 |
Species: | human (Homo sapiens) |
Cell type: | Burkitt lymphoma |
Origin: | established from the ascitic fluid of a 3-year-old boy with American-type Burkitt lymphoma in 1972; cells were described in the literature to be EBV-negative but EBV infectible, to carry the (8;14) translocation and TP53 mutations. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and RNA-Seq) |
Reference(s): | 14669, 14527, 18187 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing singly or in clumps in suspension; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out at ca. 0.8 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml; medium must be changed frequently to prevent acidification and the specified cell density should be complied |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 48 hours |
Harvest: | maximum density at ca. 1.5-2.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypodiploid karyotype; 45(44-46)<2n>X, -Y, del(2)(p16p23), t(8;14)(q24;q32.2), add(14)(p11), der(16)t(7;16)(q11.2;p13.2), der(17)t(13;17)(q33;p11.3)t(13;3)(q13;q?25), del(20)(q12); carries t(8;14) effecting IGH-MYC rearrangement |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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