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| Cell line: | WSU-FSCCL |
| DSMZ no.: | ACC 612 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established from the peripheral blood of a 37-year-old white man with low-grade follicular small cleaved cell lymphoma (B-NHL, stage IVB) after chemotherapy and transformation to small cell non-cleaved non-Hodgkin´s lymhoma in 1989; described in the literature to carry t(14;18) and t(8;11) with BCL2 and MYC rearrangements, respectively |
| Reference(s): | 14775, 14969, 14774 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polymorph cells growing singly in suspension; image; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5-1.0 x 106 cells/ml; maintain at 0.25-4.0 x 106 cells/ml; optimal split ratio of 1:3 to 1:5 about 2-3 times a week |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20-30 hours |
| Harvest: | maximum density at ca. 4.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy; 47(46-50)<2n>XY, -13, +2mar, add(1)(p34), del(6)(q25), t(8;11)(q24.3;q24), i(13q), t(14;18)(q32;q21); t(14;18) effects IGH-BCL2 rearrangement |
| Molec. Genetics: | expression of fusion gene IGH-BCL2 (MBR) confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
