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| Cell line: | BC-1 |
| DSMZ no.: | ACC 677 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established from the malignant effusion samples of a 46-year-old man with AIDS-related primary effusion lymphoma (PEL, stage IE-B) at diagnosis in 1992; cells are described in the literature to be positive for human herpesvirus type 8 (HHV-8) and Epstein-Barr virus (EBV) |
| Reference(s): | 15928, 14483, 15929, 16559, 16560 |
| Biosafety level: | 2 |
| Risk assessment: | The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is also positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions: | A, B |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly and in small clusters in suspension; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at ca. 0.5-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-4 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 35-48 hours |
| Harvest: | cell harvest of ca. 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, cyCD79a -, CD80 -, CD138 +, smIgG -, smIgM -, smkappa -, smlambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype; 50(47-51)<2n>XY, +7, +8, +8, +mar, der(3)ins(3;1)(q28;q11q42), inv(8)(p23q24)x2, der(9)t(2;9)(q32;q35), del(12)(q24), der(15)t(4;15)(q31;p13), der(16)t(15;16)(p13;q15); resembles published karyotype |
| Viruses: | PCR: EBV +, HBV -, HCV -, HHV-8 +, HIV -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
