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| Cell line: | CML-T1 |
| DSMZ no.: | ACC 7 |
| Species: | human (Homo sapiens) |
| Cell type: | T cell leukemia |
| Origin: | established from the peripheral blood of a 36-year-old woman with chronic myeloid leukemia (CML) in blast crisis in 1987; cells were described in the literature to express T cell surface markers and to carry the BCR-ABL1 translocation e13-a2 (b2-a2) producing the p210 BCR-ABL protein. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 14972, 14699, 14479, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, round cells in suspension; image; image; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml in a small vessel (a T25 flask or a 12/24-well-plate), maintain at 0.1-1.0 x 106 cells/ml; split 1:2 to 1:4 every 2-4 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48-60 hours |
| Harvest: | maximal density at 2.5-3.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD2 -, cyCD3 +, CD3 (+), CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta (+), TCRgamma/delta -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near tetraploid karyotype with diploid sideline - 92<4n>XXX, t(6;7)(q24;q35)x2, del(11)(q22.3)x2 |
| Molec. Genetics: | expression of fusion gene BCR-ABL1 (e13-a2) confirmed by RT-PCR |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
