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Cell line: | KYAE-1 |
DSMZ no.: | ACC 703 |
Species: | human (Homo sapiens) |
Cell type: | esophageal adenocarcinoma |
Origin: | established from the pleural effusion of a 60-year-old man with a distal non-Barrett-esophageal adenocarcinoma in 2001; the patient was pre-treated with radiation and chemotherapy; cells were shown to be tumorigenic in mice and to carry a TP53 mutation (C455T) |
Reference(s): | 16055, 16451 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelial cells growing adherently as monolayer and small clusters |
Medium: | 40% Ham's F12 + 40% RPMI 1640 + 20% h.i. FBS + 1 mM sodium pyruvate + 2 mM L-glutamine |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2 flask; cells do not grow to confluency; split sub-confluent culture 1:2 to 1:5 once a week using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 3-4 days |
Harvest: | cell harvest of ca. 10 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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