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Cell line: | HG-3 |
DSMZ no.: | ACC 765 |
Species: | human (Homo sapiens) |
Cell type: | chronic B cell leukemia |
Origin: | established in 1998 from leukemic B-cells of a 70-year-old man with chronic lymphocytic leukemia at diagnosis (CLL, Rai stage II). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and RNA-Seq) |
Reference(s): | 19172, 19507, 18187 |
Biosafety level: | 1 |
Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1.
According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing partly in clumps in suspension and slightly adherent; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.2 - 0.5 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50-60 hours |
Harvest: | maximum density at ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3-, CD10-, CD13-, CD19(+), CD20, CD33-, CD34-, CD37+, CD38+, CD80+, CD138(+), HLA-DR+, sIgkappa-, sIglambda+, cIgG-, cIgM+, cIgkappa-, cIgkappa+; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-diploid karyotype; 46(44-46)<2n>XY, der(10)t(10;11)(q26;q24.1), del(13)(q12q32); carries del(13) associated with CLL; marginal resemblance to original karyotype |
Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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