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| Cell line: | U-2946 |
| DSMZ no.: | ACC 795 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established in 1996 from the pleural effusion of a 52-year-old man with diffuse large B cell lymphoma (DLBCL). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s): | 18124, 18137, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly or in small clumps in suspension; image; image; image |
| Medium: | 85-90% RPMI 1640 + 10-15% h.i. FBS |
| Subculture: | seed out at ca. 0.1-0.3 x 106 cells/ml; optimal split ratio 1:5 - 1:10 every 3-4 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20-30 hours |
| Harvest: | maximum density at ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD5 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype; -47(43-48)<2n>XY, +7, der(8)del(8)(p22.2)t(8:14)(q24;q32), der(9)t(2;9)(p25:p21), der(14)t(8;14)(q24;q32), der(17)del(17)(p11.2)del(17)(q21q22); sdl with del(9)(q11); carries t(8;14) effecting IGH-MYC rearrangement; der(17) carries 1q21-derived focal amplicon inserted at 17p11 effecting MCL1 activation |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
