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Cell line: | MBU-T |
DSMZ no.: | ACC 799 |
Species: | human (Homo sapiens) |
Cell type: | keratinocytes |
Origin: | established in 2011 from primary keratinocytes of a healthy neonatal foreskin; cells were immortalized by transduction with retroviral vectors containing human telomerase, SV40 early region, and epitope-tagged mutated HRAS(V12) linked to IRES-driven GFP expression |
Reference(s): | 25648 |
Biosafety level: | 2, GMO-S2 |
Risk assessment: | Primary keratinocyte cells isolated from a neonatal foreskin were transduced with replication deficient amphotropic retroviral vectors containing a) the catalytic subunit of the human telomerase reverse transcriptase hTERT, b) SV40 early region, and c) constitutively active mutated HRASV12 genes for immortalization. Mutated HRAS exhibits strong oncogenic potential and is assigned to biosafety level 2. |
Permissions and restrictions: | A, B, D |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid cells growing in monolayer; image; image |
Medium: | 100% Keratinocyte SFM + 0.2 ng/ml hEGF + 15 µg/ml pituitary extract |
Subculture: | seed out at ca. 0.5 - 1 x 106 cells/80 cm2 flask; split 1:2 - 1:4 every 3-4 days using Accutase |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 50-60 hours |
Harvest: | cell harvest of ca. 4-6 x 106 cells/80 cm2 |
Storage: | frozen with 90% Keratinocyte SFM, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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