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| Cell line: | MCA-B1 |
| DSMZ no.: | ACC 828 |
| Species: | canine (dog) (Canis lupus familiaris) |
| Cell type: | acanthomatous epulis |
| Origin: | odontogenic tumor of the gingiva (acanthomatous epulis); established in 1992 from the tumor tissue from a 10-year old domestic dog (Akita bred, Japan) |
| Reference(s): | 27645, 27648 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently in monolayers with pavement-like colonies; image;image; image |
| Medium: | mixture of Ham's F12 + DMEM (1:1) + 5-10% h.i. FBS |
| Subculture: | seed out 1-2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 24 hours |
| Harvest: | cell harvest of 6-8 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Canis lupus familiaris species. |
| Viruses: | PCR: MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
