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| Cell line: | COR-L23R |
| DSMZ no.: | ACC 883 |
| Species: | human (Homo sapiens) |
| Cell type: | large cell lung carcinoma |
| Origin: | multidrug-resistant subclone of COR-L23 (DSMZ ACC 882), which was established in 1985 from a pleural effusion of a 62-year-old male with a giant cell anaplastic carcinoma of the lung; COR-L23R was developed by pulsing the parental cell line with 0.02 µg/ml doxorubicin (adriamycin); cells are described to form tumors in nude mice and to express MRP |
| Reference(s): | 18225, 18228, 18241, 18242, 18243, 18244 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | large epithelioid, often multinucleate cells growing adherently as monolayer; image; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS + 0.02 µg/ml Doxorubicin |
| Subculture: | seed out at about 1-2 x 106 cells in a 80 cm2 flask; split confluent cultures 1:3 - 1:5 every 2-3 days using trypsin/EDTA; after thawing omit doxorubicin, but add the drug with first passaging |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 35-40 hours |
| Harvest: | cell harvest of ca. 15 x 106 cells/80 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Molec. Genetics: | expression of ABCC1 (encoding MRP) was confirmed by qPCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
