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| Cell line: | BL-30 |
| DSMZ no.: | ACC 916 |
| Species: | human (Homo sapiens) |
| Cell type: | Burkitt lymphoma |
| Origin: | established in 1982 from bone marrow of a 19-year-old male with Burkitt lymphoma in relapse; cells are EBV-negative, contain a mutation in exon 6 of TP53 and were described to carry a t(8;14) translocation; a matched B-lymphoblastoid cell line is available (IARC-139, DSMZ ACC 924) |
| Reference(s): | 40234, 40224, 14434, 40225, 40226, 14714 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | mostly round cells growing singly and partially in small aggregates in suspension
; image; image; image |
| Medium: | 95% RPMI 1640 + 5% h.i. FBS |
| Subculture: | seed out at about 0.1-0.5 x 106 cells/ml; split 1:5 to 1:10 every 2-4 days and maintain at 0.2-0.5 x 106 cells/ml (densities above 1 x 106 cells/ml will induce apoptosis) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | varies between 15-24 hours depending on cell density |
| Harvest: | maximal density of ca. 1 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD30 -, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, sm/cy IgM +, sm/cy IgG -, sm/cy kappa -, sm/cy lambda +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database; matching autologous IARC-139. |
| Molec. Genetics: | a TP53 mutation in exon 6 (737T>C) was verfied by RT-PCR (while the corresponding B-LCL is wild type) |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
