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Cell line: | 3T3 |
DSMZ no.: | ACC 173 |
Species: | murine (mouse, Swiss albino mouse) (Mus musculus) |
Cell type: | fibroblasts |
Origin: | established from disaggregated Swiss albino mouse embryos in 1962; cells are used for transfection studies with DNA viruses and as assay system for transformation studies; cells are described to have a high sensitivity to contact inhibition |
Reference(s): | 15016, 15015 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | fibroblasts growing adherently as monolayer with contact inhibition; image; image; image; image; image;image |
Medium: | 90-95% DMEM + 5-10% h.i. FBS |
Subculture: | split confluent culture at a ratio of up to 1:8 every 2 to 4 days using trypsin/EDTA; after trypsination cells might remain in aggregates that are difficult to disperse; upon reaching confluence cells stop to grow; cells grow fast at low titer; seed out at ca. 1-2 x 106 cells/175 cm2 |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 40 hours |
Harvest: | cell harvest of about 5-8 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | murine hypertriploid (near tetraploid sideline present) karyotype with 13% polyploidy - 68(58-73)<3n> |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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