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Cell line: | AN3-CA |
DSMZ no.: | ACC 267 |
Species: | human (Homo sapiens) |
Cell type: | endometrial adenocarcinoma |
Origin: | established in 1962 from the lymph node metastasis resected from a 55-year-old white woman with an endometrial adenocarcinoma (epithelial undifferentiated small cell type) presenting with acanthosis nigricans; forms heterotransplantable tumors |
Reference(s): | 14448 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial cells growing as adherent monolayer; prominent cytoplasmic vesicles; image; image |
Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS + 1 mM sodium pyruvate and non-essential amino acids |
Subculture: | split confluent culture 1:3 to 1:8 once or twice weekly using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/25 cm2 |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 55 hours |
Harvest: | cell harvest of about 10-15 x 106 cells/75 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 14% polyploidy - 49(45-50)<2n>XX, +5, +6, +7, der(3)t(1;3)(q24;p22) |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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