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DSM No.: | 115302 dsm-115302 dsm115302 dsm 115302 Acanthamoeba hatchetti |
Strain designation: | AH3 |
Isolated from: | Activated sludge samples from the surface of a nitrifying reactor in a wastewater treatment plant |
Country: | Austria Klosterneuburg |
Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. |
History: | <- P. Arthofer, University of Vienna, Centre for Microbiology and Environmental Systems Science, Vienna, Austria; AH3 {2017} |
Genbank accession numbers: | 18S rRNA gene: ON532723.1 draft genome (raw reads): SRX13852144 |
Cultivation conditions: | Medium 1796 , 20°C
Acanthamoeba hatchetti AH3 DSM 115302 is the host of the obligate intracellular bacterium Parachlamydia acanthamoebae strain PAVD. The infected culture of DSM 115302 Acanthamoeba hatchetti AH3 (8 ml) is maintained in 25 cm2 tissue culture flasks at 20 °C in DSMZ Medium 1796 in the dark. As food source E. coli K12 DSM 498 is used. The culture is transferred weekly. Therefore, 1 ml of the culture is transferred to fresh 7 ml DSMZ Medium 1796 supplemented with 100 µl E. coli K12 DSM 498 with an optical density of OD600 = 30.
Complete DSMZ Media List |
Summary and additional information: | <- P. Arthofer, University of Vienna, Centre for Microbiology and Environmental Systems Science, Vienna, Austria; AH3 {2017}. Activated sludge samples from the surface of a nitrifying reactor in a wastewater treatment plant. Austria, Klosterneuburg. Sequence accession no. 18S rRNA gene: ON532723.1, draft genome (raw reads): SRX13852144. Strain-specific literature (40697). (Medium 1796, incubation time: 3-7 days, pH 7, 20°C, aerobic). Acanthamoeba hatchetti AH3 DSM 115302 is the host of the obligate intracellular bacterium Parachlamydia acanthamoebae PAVD. The infected culture of DSM 115302 Acanthamoeba hatchetti AH3 (8 ml) is maintained in 25 cm2 tissue culture flasks at 20 °C in DSMZ Medium 1796 in the dark. As food source E. coli K12 DSM 498 is used. The culture is transferred weekly. Therefore, 1 ml of the culture is transferred to fresh 7 ml DSMZ Medium 1796 supplemented with 100 µl E. coli K12 DSM 498 with an optical density of OD600 = 30. |
Literature: | 40697 |
Risk group: | 2 (classification according to German TRBA) |
Restrictions: | Act dealing with the prevention & control of infectious diseases in man (Infektionsschutzgesetz), Category A1
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Supplied as: | Delivery form | | Prices | | actively growing culture | | 160,- € | | DNA | | 150,- € | | Price Category for this culture: 1 |
Freight and handling charges will be added. See price list. Note: Freight charges for Risk Group 2 microorganisms will apply. |
Other cultures: | All DSMZ cultures of the species |
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