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CYANO | How to read the following data (Example) |
Name: | "Coleofasciculus sp." |
DSM No.: | 104256 dsm-104256 dsm104256 dsm 104256 Coleofasciculus sp. |
Strain designation: | ASK5 |
Isolated from: | Brackish habitat (9 PSU) |
Country: | Sweden Baltic Sea, Askø |
Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. |
History: | <- U. Karsten, Universität Rostock, Germany; {2014} <- K. Witte and St. Forster, Universität Rostock, Germany; ASK5 {2002} |
Genbank accession numbers: | 16S + 23S partial sequence, intergenic spacer: EF654026 16S + 23S partial sequence, intergenic spacer: OR297941 |
Cultivation conditions: | Medium 1593 , alternative medium: DSMZ Medium 1680, 17°C
At the DSMZ, the cyanobacterial cultures are usually cultivated without agitation in 25 cm2 plastic tissue culture flasks with filter screw caps. To subculture Coleofasciculus strains, scrape off the adhering cell material with a plastic Pasteur pipette and crush it by pipetting up and down several times. Alternatively, use a disposable pipette tip to dissect the cell material. Use 2-3 ml of the shredded biomass to inoculate 15 ml of fresh medium in a new tissue culture flask. Light cycle: 16 hours of illumination at 300-350 lux and 8 hours dark period. Transfer interval of 12 weeks.
Complete DSMZ Media List |
Summary and additional information: | <- U. Karsten, Universität Rostock, Germany; {2014} <- K. Witte and St. Forster, Universität Rostock, Germany; ASK5 {2002}. Brackish habitat (9 PSU). Sweden, Baltic Sea, Askø. Sequence accession no. 16S + 23S partial sequence, intergenic spacer: EF654026, 16S + 23S partial sequence, intergenic spacer: OR297941. Phototrophic. It cannot be excluded that the strain produces cyanobacterial toxins that can be dangerous to humans and pets. DSM 104256 is a non-axenic culture. Strain-specific literature (27474). (Medium 1593, incubation time: more than 4 weeks, 17°C, alternative medium: DSMZ Medium 1680). At the DSMZ, the cyanobacterial cultures are usually cultivated without agitation in 25 cm2 plastic tissue culture flasks with filter screw caps. To subculture Coleofasciculus strains, scrape off the adhering cell material with a plastic Pasteur pipette and crush it by pipetting up and down several times. Alternatively, use a disposable pipette tip to dissect the cell material. Use 2-3 ml of the shredded biomass to inoculate 15 ml of fresh medium in a new tissue culture flask. Light cycle: 16 hours of illumination at 300-350 lux and 8 hours dark period. Transfer interval of 12 weeks.
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Risk group: | 1 (classification according to German TRBA) |
Supplied as: | Delivery form | | Prices | | actively growing culture | | 160,- € | | Price Category for this culture: 1 |
Freight and handling charges will be added. See price list. |
Other cultures: | All DSMZ cultures of the species |
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