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| Cell line: | A-427 |
| DSMZ no.: | ACC 234 |
| Species: | human (Homo sapiens) |
| Cell type: | lung carcinoma |
| Origin: | established from the lung adenocarcinoma of a 52-year-old man; well characterized cell line (see Cellosaurus:Expasy) and a complex karyotype; listed in the CCLE (ref 18232) |
| Reference(s): | 14556, 14861, 40412, 18232 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like adherent cells growing in monolayers; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | split confluent culture 1:3 every 4-5 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2; after thawing, recovery may be slow and adherence may be reduced |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 60-70 hours |
| Harvest: | cell harvest of about 25 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near triploid karyotype with 2% polyploidy - 67(59-72)<3n>XXY, +3, +7, -13, -14, -15, -18, -19, -20, -22, +4mar, add(1)(p11), del(1)(q11), del(3)(p11)x2, der(3)t(1;3)(q21;q11), add(4)(q31), del(6)(q21), del(7)(p11), i(10q), add(11)(q24), add(14)(p11), add(15)(p13), add(18)(q23) - 3p- deletion is characteristic of lung carcinoma - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
