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| Cell line: | ARH-77 |
| DSMZ no.: | ACC 512 |
| Species: | human (Homo sapiens) |
| Cell type: | B lymphoblastoid cells |
| Origin: | established from the peripheral blood of a 33-year-old woman with IgG plasma cell leukemia; cell line is widely used as model for multiple myeloma/plasma cell leukemia, but cells are clearly EBV+ indicating that ARH-77 might be rather a "normal" B-lymphoblastoid cell line than a tumor cell line |
| Reference(s): | 14471, 14472, 14844, 14486 |
| Biosafety level: | 1 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round single cells or clustered growing in suspension, some cells loosely adherent; image; image |
| Medium: | 90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; start culture in 24-well-plates; maintain at 0.2-1.5 x 106 cells/ml and split saturated culture 1:2 to 1:5 every 2-3 days; adherent cells can be detached by tapping the flask |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | saturation density at ca. 1.5-2.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | during accessioning contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture and continuosly in PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD71 +, cyCD79 +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near diploid karyotype with 8% polyploidy - 46(44-48)<2n>XX, +9, del(1)(q23), add(2)(q21), add(3)(p11), der(3)t(2;3)(q23;q26), del(6)(p21), der(9)t(9;17)(q10;q10) - sideline with der(X)t(X;1)(q23;p32), del(16)(p13.2) and unresolved der(3) and der(9)hsr markers - no IGH translocations detected |
| Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
