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established from the peripheral blood of a 33-year-old woman with IgG plasma cell leukemia; cell line is widely used as model for multiple myeloma/plasma cell leukemia, but cells are clearly EBV+ indicating that ARH-77 might be rather a "normal" B-lymphoblastoid cell line than a tumor cell line
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
round single cells or clustered growing in suspension, some cells loosely adherent; image; image
90% RPMI 1640 + 10-20% h.i. FBS
seed out at ca. 0.5 x 106 cells/ml; start culture in 24-well-plates; maintain at 0.2-1.5 x 106 cells/ml and split saturated culture 1:2 to 1:5 every 2-3 days; adherent cells can be detached by tapping the flask
at 37 °C with 5% CO2
ca. 30 hours
saturation density at ca. 1.5-2.0 x 106 cells/ml
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
during accessioning contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture and continuosly in PCR assays
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
human near diploid karyotype with 8% polyploidy - 46(44-48)<2n>XX, +9, del(1)(q23), add(2)(q21), add(3)(p11), der(3)t(2;3)(q23;q26), del(6)(p21), der(9)t(9;17)(q10;q10) - sideline with der(X)t(X;1)(q23;p32), del(16)(p13.2) and unresolved der(3) and der(9)hsr markers - no IGH translocations detected