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Cell line: | BEL-1 |
DSMZ no.: | ACC 968 |
Species: | human (Homo sapiens) |
Cell type: | B cell precursor leukemia |
Origin: | established in 2004 from periphal blood of a 41-year-old-female patient with relapsed B-cell acute lymphoblastic leukemia (B-ALL); cells are described to show a pro B-cell immunophenotype and carry t(4;11)(q21;q23) resulting in KMT2A::AFF1 fusion |
Reference(s): | 40761 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing singly and in small clumps in suspension; image; image; image |
Medium: | 80-90% RPMI 1640 + 10-20% FBS |
Subculture: | seed out at about 0.5 x 106 cells/ml in a 24-well plate; split 1:2 to 1:3 every 2-3 days; maintain at 0.5-1 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 48 hours |
Harvest: | max density of 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD5 -, CD10 -, CD13 -, CD19 +, CD20 -, CD33 -, CD37 (+), CD38 +, CD80 -, HLA-DR +, sm kappa +, sm IgG -, sm IgM -, cy IgG +, cy lambda +
; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Molec. Genetics: | fusion gene KMT2A::AFF1 was verified by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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