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| Cell line: | YCUB-2 |
| DSMZ no.: | ACC 961 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell precursor leukemia |
| Origin: | Established in 1997 from a peripheral blood sample of a 4-year old boy with B-cell acute lymphoblastic leukemia (B-ALL) at relapse; cells carry a TCF3::HLF fusion gene deriving from t(17;19) chromosomal translocation |
| Reference(s): | 40755, 40758, 40759 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polymorph cells, growing as single cells in suspension; image; image |
| Medium: | 90% RPMI 1640 + 10% FBS |
| Subculture: | seed out at about 0.5 x 106 cells/ml; split
1:3 to 1:8 every 2-3 days; optimal cell density 0.5-2.5 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 24 hours |
| Harvest: | max density of 3 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD2 -, CD3 -, CD5 -, CD10 +, CD13 -, CD19 +, CD20 -, CD25 -, CD33 +, CD34 -, CD37 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm IgG -, sm IgM -, sm/cy kappa -, sm/cy lambda -, cy IgG -, cy IgM +, cy CD79a (+); image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Molec. Genetics: | fusion gene TCF3::HLF was verified by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
