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| Cell line: | BL-60 |
| DSMZ no.: | ACC 953 |
| Species: | human (Homo sapiens) |
| Cell type: | Burkitt lymphoma |
| Origin: | established in 1984 from the maxillary biopsy of a 4-year-old girl with EBV-positive Burkitt lymphoma; described to carry a t(8;22) chromosomal translocation |
| Reference(s): | 40773, 40252, 40431, 40767, 40774, 40784 |
| Biosafety level: | 2 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | large round and small polygonal cells in suspension, partially growing in aggregates; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 48 hours |
| Harvest: | max density of 3 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 (+), CD138 +, HLA-DR +, sfIgG +, sfIgM +, sfIgKappa -, sfIgLambda +, cyIgG -, cyIgM +, cyIgKappa +, cyIgLambda +, cyCD79a (+); image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database |
| Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
