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| Cell line: | EFM-192A |
| DSMZ no.: | ACC 258 |
| Species: | human (Homo sapiens) |
| Cell type: | breast carcinoma |
| Origin: | established from the pleural effusion of the left side from a 46-year-old woman with invasive breast adenocarcinoma in 1985; sister cell lines EFM-192B and C show differences in molecular aspects; erbB-2 gene is amplified and overexpressed;
RNA sequence data are available (see Ref 40906 and RNA-Seq) |
| Reference(s): | 15156, 40906 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid, adherent growing as monolayer in compact colonies; image; image; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at ca. 2 x 106 cells/25 cm2; split confluent culture 1:2 to 1:4 once a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 5-8 days |
| Harvest: | cell harvest of about 8-12 x 106 cells/ 80cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded near-triploid karyotype with 20% polyploidy - 68(60-70)<3n>XXXX, +X, -1, -4, +9, -12, -17, -17, -18, -22, +6mar, add(1)(p22), add(1)(p?32), add(2)(q35/37), add(3)(q27/29), i(15q), add(17)(p12), dminx1 present at 38% |
| Molec. Genetics: | no or weak expression of ER, PR, AR but strong expression of HER2 as determined by Western blot |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
