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| Cell line: | CAL-27 |
| DSMZ no.: | ACC 446 |
| Species: | human (Homo sapiens) |
| Cell type: | tongue squamous cell carcinoma |
| Origin: | established from the poorly differentiated squamous cell carcinoma of the tongue removed from a 56-year-old man before treatment in 1982 |
| Reference(s): | 14562 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing adherently in monolayer; image; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | seed out 1-2 x 106 cells in a small (25cm2) culture flask; split confluent culture 1:4 to 1:10 every 3-6 days using trypsin/EDTA; maximum density of ca. 14-18 x 106 cells/80cm2 flask |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 20-40 hours |
| Harvest: | cell harvest of ca. 8-12 x 106 cells/80cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypodiploid karyotype with 3% polyploidy - 41(38-45)<2n>X, -Y, -4, +7, -10, -11, -18, -20, -21, -22, +2-3mar, add(3)(q21), i(3q), i(7p), del(8)(p21), i(9q), add(13)(p11), add(14)(p11) - sideline with additional der(3) and der(7) - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
