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| Cell line: | BL-64 |
| DSMZ no.: | ACC 919 |
| Species: | human (Homo sapiens) |
| Cell type: | Burkitt lymphoma |
| Origin: | established in 1982 from the bone marrow of a 4-year-old boy with Burkitt lymphoma in relapse; cells are EBV-positive and were described to carry a t(2;8) translocation leading to MYC-IgK fusion; a matched B-lymphoblastoid cell line is available (IARC-549, DSMZ ACC 927) |
| Reference(s): | 40234, 40314, 40315, 40316 |
| Biosafety level: | 2 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in phorbol ester / sodium butyrate stimulated cells. The infection was classified as potentially lytic associated with a production of active viruses. A transmission of EBV during handling of the cells cannot be excluded and the cell line is therefore classified biosafety level 2. |
| Permissions and restrictions: | A, B |
| | DSMZ Cell Culture Data: |
| Morphology: | mostly round cells growing singly, partly in aggregates in suspension, also some single giant cells; image; image; image |
| Medium: | 90% Iscove's MDM + 10% h.i. FBS + 100 µM Thioglycerol |
| Subculture: | seed out at about 0.3 x 106 cells/ml; split
1:3 to 1:10 every 2-4 days; maintain at 0.2 x 106 - 0.5 x 10 6 cells/m |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 30-50 hours, depending on cell density |
| Harvest: | maximal density of ca. 1 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD30 -, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, cyIgM +, cyIgG -, cykappa +, cylambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching autologous IARC-549 |
| Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
