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| Cell line: | F9 |
| DSMZ no.: | ACC 112 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | embryonal carcinoma |
| Origin: | initiated from a testicular teratocarcinoma of a strain 129 mouse |
| Reference(s): | 14386, 14989 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent cells with undifferentiated morphology growing in clumps; image; image |
| Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture: | presoak flasks with 0.1% gelatin at 4 °C for 2 hours, wash 3x with PBS; split confluent culture 1:10 to 1:20 every 2-4 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 24 hours |
| Harvest: | about 70 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine near-diploid karyotype with 6% tetraploidy - 38(37-40)<2n> - 1-2 centric fusion markers present |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
