| |
---|
Cell line: | CL-40 |
DSMZ no.: | ACC 535 |
Species: | human (Homo sapiens) |
Cell type: | colon carcinoma |
Origin: | established from the primary colorectal cancer (TNM stage 3) of the right colon of a woman; cells were described in the literature to be well differentiated |
Reference(s): | 14528, 40608 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent epithelial-like cells growing as monolayers and in aggregates; image; image |
Medium: | 80% mixture of DMEM + Ham's F12 at 1:1 + 20% h.i. FBS |
Subculture: | seed out at 1-2 x 106 cells/75 cm2; split confluent culture 1:4 once a week using the double amount of trypsin/EDTA for up to 20 min (cells are difficult to detach); reduced viability immediately after thawing (ca. 50%), cells recover subsequently over the next weeks |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 3-4 days |
Harvest: | cell harvest of ca. 10-12 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 8% polyploidy - 48(45-49)<2n>XX, +12, +13 - no consistent structural abnormality was detected |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |