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| Cell line: | CL-50IIa |
| DSMZ no.: | ACC 623 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | cholangio-hepatocellular tumor |
| Origin: | established ca. 1986 from the liver of a diethylnitrosamine-treated male Wistar AF/Han rat |
| Reference(s): | 14658, 14992 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing adherently in monolayers; image; image; image |
| Medium: | 80-90% MEM (with Earle's salts) + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2; optimal split ratio 1:3 every 3-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-60 hours |
| Harvest: | maximum density at ca. 6 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
