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| Cell line: | COLO-720L |
| DSMZ no.: | ACC 206 |
| Species: | human (Homo sapiens) |
| Cell type: | B lymphoblastoid cells (from patient with adenocarcinoma) |
| Origin: | B-lymphoblastoid cell line established from the peripheral blood of a 73-year-old woman with metastatic adenocarcinoma in 1988 |
| Reference(s): | not published |
| Biosafety level: | 1 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | lymphoblast-like cells growing in suspension as single cells and in clumps; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | maintain at 0.5-1.0 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 (or may be grown in closed flasks) |
| Doubling time: | ca. 20-30 hours |
| Harvest: | saturation density at about 2.0-2.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD37 +, HLA-DR +, sm/cyIgM -, smIg/cyG+, sm/cykappa (+)/+, sm/cylambda - |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 10% polyploidy - 47(45-50)<2n>XX, +12, der(5)t(3;5)(p21.3;p15) - the 3p21.3 breakpoint is associated with benign adenoma |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
