| | |
|---|
| Cell line: | CX-03 |
| DSMZ no.: | ACC 816 |
| Species: | human (Homo sapiens) |
| Cell type: | uterine carcinosarcoma |
| Origin: | established in 2015 after xenotransplantation in SCID-mice of primary tumor cells from a mixed Muellerian tumor (Carcinosarcoma) of the uterus of a 49-year-old woman; cells are described in literature to produce mesothelin and mucus |
| Reference(s): | 18179 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently in monolayers |
| Medium: | 80% DMEM + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture 1:2 -1:4 every 7 days using trypsin/EDTA; cells grow rather slowly in the first two week after thawing |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 4-7 days |
| Harvest: | cell harvest of ca. 4 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
