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Cell line: | DG-75 |
DSMZ no.: | ACC 83 |
Species: | human (Homo sapiens) |
Cell type: | Burkitt lymphoma |
Origin: | established from the pleural effusion of a 10-year-old boy with Burkitt lymphoma (refractory, terminal) in 1975 |
Reference(s): | 14377 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round to polygonal, single or clustered cells in suspension; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.1-0.5 x 106 cells/ml; split 1:5 to 1:10 every 24-48 hours |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 40-50 hours |
Harvest: | saturation density at 0.75-1.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, cyIgG -, cyIgM +, cykappa +, cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near diploid karyotype with 6% polyploidy - 46<2n>XY, t(8;14)(q24;q32) |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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